. 2017 Dec 21;8:2576. doi: 10.3389/fmicb.2017.02576

Table 1.

Bacterial strains and plasmids used in this study.

Strain/plasmid	Description	Relevant phenotype	Source/References
STRAINS
E. coli
HB101	F⁻Δ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 xyl-5 mtl-1 recA13	Str^R, $r_{B}^{-}$ $m_{B}^{-}$	Sambrook and Russell, 2001
XL1-Blue	F'::Tn10 proA⁺B⁺ lacI^q Δ(lacZ)M15/recA1 endA1 gyrA96 (Nal^R) thi hsdR17 ( $r_{K}^{-}$ $m_{K}^{+}$ ) supE44 relA1 lac		Stratagene
R. capsulatus
MT1131^a	crtD121 Rif ^R	Wild type, Res⁺ Nadi⁺ Ps⁺^b	Scolnik et al., 1980
SB1003	Wild type	Wild type, Res⁺ Nadi⁺ Ps⁺	Yen and Marrs, 1976
Y262		GTA overproducer	Yen et al., 1979
MD20	Δ(dsbA::kan)	Res⁺ Nadi⁺ Ps⁺ on Med A^c Res^Ts Nadi⁻ Ps⁺ on MPYE^c	Deshmukh et al., 2003
ST20	Δ(dsbA::spe)	similar to MD20 but Spe^R	This work
SE8	Δ(ccoA::spe)	Res⁺ Nadi⁻ Ps⁺ on MPYE Res⁺ Nadi⁺ Ps⁺ on MedA	Ekici et al., 2012a
GK32	Δ(ccoNO::kan)	Res⁺ Nadi⁻ Ps⁺	Koch et al., 1998
KZ1	Δ(Q_ox::spe)	Res⁺ Nadi⁺ Ps⁺	Zhang and Daldal
LS01	Δ(senC) derivative of SB1003	Res⁺ Nadi⁻ Ps⁺ on MPYE Res⁺ Nadi⁺ Ps⁺ on MedA	Swem et al., 2005
DS1	Δ(dsbA::kan) Δ(Q_ox::spe) derivative of KZ1	Like MD20, except Ps⁺ Res⁻ on all media	This work
OZL4	Δ(dsbA::spe) Δ(ccoNO::kan) derivative of GK32	Like MD20, except Res⁺ Nadi⁻ on all media	This work
PLASMIDS
pRK2013	tra⁺ (RK2)	Kan^R, helper	Ditta et al., 1985
pRK415	Broad host-range vector	Tet^R	Keen et al., 1988
pBluescript	Cloning vector, pBluescript II KS (+)	Amp^R	Stratagene
pHP45ΩSpe	Ωspe in pHP45 vector	Spe^R, Amp^R	Prentki and Krisch, 1984
pTC4-1K	4.2 kb XbaI-KpnI insert carrying dsbA::kan allele cloned into the corresponding sites of pRK415	Kan^R, Tet^R	Deshmukh et al., 2003
pKZ2	Δ(Qox::spec) allele in pRK415	Spe^R, Tet^R	Zhang and Daldal
p4AiK32	Δ(ccoNO::kan) in pRK415	Kan^R, Tet^R	Koch et al., 1998
pOX15	Wild type ccoNOQP in pRK404	Tet^R	Koch et al., 1998
pDsbA^wt	Wild type dsbA carried by pRK415	Tet^R	Deshmukh et al., 2003
pBK69	Wild type ccoA carried by pRK415-pBAD	Amp^R, Tet^R	Khalfaoui-Hassani et al., 2016a
pSenCFlagC	Wild type senC with a C-ter Flag epitope carried by Prkm	Kan^R	Onder et al., 2008

R. capsulatus strain MT1131 was originally isolated as a “green” (i.e., carrying crtD121 mutation) derivative of R. capsulatus wild type SB1003 (Scolnik et al., 1980). All mutants are derivative of MT1131 unless mentioned otherwise.

Ps and Res refer to photosynthetic and respiratory growth ability, respectively. Nadi indicates Cox-dependent ability to catalyze α-naphthol to indophenol blue. Res^Ts refers to the ability to grow at 25°C but not at 35°C.

MedA and MPYE refer to minimal and enriched growth media, respectively.