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. 2017 Dec 21;8:1810. doi: 10.3389/fimmu.2017.01810

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Copyright © 2017 Hendrickx, van Scheppingen, van der Poel, Bossers, Schuurman, van Eden, Hol, Hamann and Huitinga.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional role of scavenger receptors in myelin phagocytosis in vitro. MSR1, CXCL16, OLR1, and CD68 were downregulated with antisense oligonucleotides in the human macrophage cell line THP-1. Silencing efficiency was determined on mRNA level with quantitative polymerase chain reaction (A) and on protein level with immunocytochemistry [(B); only shown for MSR1]. Uptake of pHrodo-labeled myelin was validated by flow cytometry (C) and compared for myelin obtained from control and multiple sclerosis (MS) brain tissue (D). The number of cells that had phagocytosed myelin, and the total amount of myelin phagocytosed (geomean pHrodo) were calculated. The number of independent experiments (n) was 6 (MSR1), 4 (CXCL16), 3 (OLR1), and 3 (CD68). (E) Fucoidan was used to inhibit a broad spectrum of scavenger receptors in THP-1 cells. Provided is the number of cells that had phagocytosed myelin, the total amount of myelin phagocytosed (geomean pHrodo), and the viability of cells at the time point of analysis (n = 3). Scale bar in panel (B) = 200 µm *p < 0.05, **p < 0.01, ***p < 0.005.