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. 2017 Dec 21;11:415. doi: 10.3389/fncel.2017.00415

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Copyright © 2017 Parween, Varghese, Ardah, Prabakaran, Mensah-Brown, Emerald and Ansari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression changes during cortical differentiation in the presence of higher O-GlcNAc. (A) mRNA expression analysis of TBR1 gene by qRT-PCR in TMG treated and vehicle control samples from indicated time points (Day33 to Day54) of cortical differentiation using gene specific primers. The expression of β-ACTIN gene was used to normalize the expression. The bars represent normalized fold mRNA expression with values of vehicle control samples at Day22 set as 1. The data are represented as mean ± standard deviation. ^*p ≤ 0.05, ^**p ≤ 0.01, ^***p ≤ 0.001 (two-tailed unpaired Student's t-test). The data are representative of three independent biological replicates. (B) Western blot analysis of TMG treated and vehicle control samples were performed at the indicated time points (Day0 to Day54) of cortical differentiation to detect the expression of TBR1. The expression of β-ACTIN was used as loading control. (C) mRNA expression analysis of the indicated genes by qRT-PCR using TMG treated and vehicle control samples from the indicated time points (Day33 to Day54) of cortical differentiation using. The expression of β-ACTIN gene was used to normalize the expression. The bars represent normalized fold mRNA expression with values of vehicle control samples at Day22 set as 1. The data are represented as mean ± standard deviation. ^*p ≤ 0.05, ^**p ≤ 0.01, ^***p ≤ 0.001 (two-tailed unpaired Student's t-tst). The data are representative of three independent biological replicates.