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. 2017 Dec 21;8:2579. doi: 10.3389/fmicb.2017.02579

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Copyright © 2017 Tiedt, Mergelsberg, Eisenreich and Boll.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ultraperformance liquid chromatography (UPLC) diagrams demonstrating the time-dependent in vitro conversion of (A) 2-F-BzCoA and (B) BzCoA by cell-free extracts of T. aromatica. UPLC analyses of samples are shown, that were taken at representative time points as indicated by the numbers (min) at the y-axis. The peak numbers refer to those assigned to structures in Figure 1: 2-F-BzCoA (1), BzCoA (4), 1,5-dienoyl-CoA (5), 6-OH-1-enoyl-CoA (6), 3-OH-pimeloyl-CoA (8), acetyl-CoA (9). Formation of 6-oxo-1-enoyl-CoA was not observed.