FIGURE 4.

Effects of nuciferine on the secretion of cytokines. (A) The level of cytokines TNF-α, IL-6, IL-1β, and IL-10 mRNAs induced by LPS were detected qRT-PCR in tissues and cells. GAPDH served as the control. (B) The expressions of TNF-α, IL-6, IL-1β, and IL-10 were detected using an ELISA kit in tissues and cells. L and H indicate the tissues treated with 10 and 20 mg/kg nuciferine, respectively, or the cells treated with 10 and 20 μg/mL nuciferine, respectively. Data are represented as the mean ± SEM of three independent experiments. ^#p < 0.05 vs. Control group, ^∗p < 0.05 vs. LPS group.