FIGURE 7.

Effects of TLR4-siRNA transfection on LPS-induced inflammatory responses. (A) RAW264.7 cells were subjected to transfection with either control siRNA or TLR4 siRNA. The immunofluorescence technique was performed to determine TLR4 expression. Quantification of TLR4 positive density in each group. (B) After TLR4 interference, the LPS induced the production of pro-inflammatory cytokines IL-1β and TNF-α, which were measured using an ELISA kit. Data are represented as the mean ± SEM of three independent experiments. ^#p < 0.05 vs. Control group, ^∗p < 0.05 vs. LPS group.