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. 2017 Aug 7;22(1):241–250. doi: 10.1111/jcmm.13312

Figure 3.

Figure 3

TBX5 was a direct target of miR‐10a‐5p. (A) The schematic graph showed pairing relationship between miR‐10a‐5p and TBX5 mRNA 3′UTR in human. Target relationship between TBX5 mRNA 3′UTR and miR‐10a‐5p (B) was detected by dual‐luciferase reporter assay. For the luciferase reporter assay, HeLa cells were cotransfected with the luciferase reporter vectors (pMIR‐REPORT™) containing TBX5 3′UTR along with control mimic or miR‐10a‐5p mimic. After 48 hrs of transfection, relative luciferase activity was measured. HeLa cells were transfected with 50 nM miR‐10a‐5p mimics or 50 nM miR‐10a‐5p inhibitors for 48 hrs, and TBX5 expression (C and D) was measured by Western blotting analysis. The data represent the means ± S.E.M. of three independent experiments. Levels of significance were calculated using Student's t‐test (*P < 0.05, **P < 0.01).