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. 2017 Aug 7;22(1):241–250. doi: 10.1111/jcmm.13312

Figure 5.

Figure 5

TBX5 mediated the downstream inflammation in IL‐1β‐stimulated SW982 cells. SW982 cells were transfected with overexpressing plasmid pCMV3‐TBX5‐GFPSpark or siRNA‐TBX5 to up‐regulate or down‐regulate its expression levels, respectively, and both mRNA and protein expression levels of TBX5 were confirmed by RT‐qPCR and Western blotting (A and B). Then IL‐1β was used to stimulate SW982 cells which were already transfected with overexpressing plasmids or siRNAs, and expression levels of cytokines (C and E), and TLR3 and MMP13 (D and F) were detected by RT‐qPCR and Western blotting. The data represent the means ± S.E.M. of three independent experiments. Levels of significance were calculated using Student's t‐test (*P < 0.05, **P < 0.01).