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. 2017 Nov 17;22(1):521–532. doi: 10.1111/jcmm.13340

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© 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Patient 1 (Table 1). Laser confocal scanning microscopy of the sinoatrial node (SAN). (A) Blue fluorescence of cell nuclei (DAPI). (B) Green fluorescence of S100. (C) Red fluorescence of CD34. (D, E) Coexpression of CD34 and S100 on the telocytes is visualized with yellow/orange colour (arrows). A, B, C, D: ×100. E (inserted): ×900. (F) The scatterplot for the colocalization of S100 (ch2) and CD34 (ch3). (G) The intensity histograms of green (S100), red (CD34), and blue (DAPI) fluorescence and white (differential interference contrast (DIC) image. Figures F and G show the fluorescence intensity and the colocalization of the markers being studied and their quantitative levels.