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In vitro IFN‐γ production was analysed in vaccinated mice 2 weeks after the last booster by ELISpot assay. Splenocytes were harvested and treated with (+peptide) or without peptides (‐peptide) and the IFN‐γ secretion was determined. The data indicate the mean ± S.E.M. (n = 3). Statistical significances were designated as follows: ns: P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
