Figure 8.

(left) without applied flow; (right) with applied flow. CoCl₂ added on day 24. Data shows that application of 100 μmol/L CoCl₂ significantly decreased I _eq values of differentiated mCCD monolayers after 48 h (2 days). Interestingly, after 3 days of treatment, I _eq value switched to the positive direction. Current values are plotted as relative to average I _eq values as discussed in the main text. N = 4–16. One‐way ANOVA shows no significant (P > 0.05) I _eq changes among control groups in (A) or (B). * denotes significant change (P < 0.05) between a control and CoCl₂ treatment at a specific time‐point. # denotes CoCl₂ treatment causes significant change (P < 0.05) between CoCl₂‐treated sample at a specific time point with CoCl₂‐treated or control sample at a different time point. In Figure (A) and (B), CoCl₂ treatment shows gradual decrease in negative I _eq; some of this gradual decrease was statistically significant (e.g., Figure (A) Day 25 CoCl₂ vs. Day 26 CoCl₂).