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. 2017 Dec 26;5(24):e13556. doi: 10.14814/phy2.13556

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© 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of LRG on TGF‐β‐induced Smad1/5/8 phosphorylation and downstream gene expression in L929. (A) qPCR analysis of Id1. L929 cells were treated with 2 ng/mL of TGF‐β1 for 1, 3, and 6 h. The relative expression of Id1, normalized to Hprt1 gene expression is shown. (B) Detection of phospho‐Smad1/5/8 and Id1 by western blot analysis. L929 cells were transfected with endoglin siRNA and control siRNA and then treated with 2 ng/mL of TGF‐β1 for 0, 30, and 60 min. Endoglin, phospho‐Smad1/5 (Ser463/465)/8 (Ser426/428), Id1, LRG, and GAPDH were detected by western blot analysis.