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. 2017 Sep 18;22(1):497–510. doi: 10.1111/jcmm.13338

Figure 1.

Figure 1

LncRNA PLAC2 is strongly down‐regulated in glioma tissues and inhibits glioma cell proliferation, induces cell cycle arrest. (A) PLAC2 was down‐regulated in glioma as compared to normal brain tissues. Log10 transformation was applied to expression levels. *P < 0.05, **P < 0.01. (B) Representative images of PLAC2 expression in glioma (Tumour) and adjacent non‐cancerous tissue by FISH (n = 6). (C) Percentage of nuclear and cytoplasmic RNA levels of PLAC2 and GAPDH measured by qRT‐PCR after cell fractionation in U87MG cells. The graph shows the average of three independent experiments. (D) Stable overexpression of PLAC2 in U87MG and U251 cells after lentiviral infection with LV‐PLAC2. Control cells were infected with the empty lentiviral vector LV‐Mock. Experiments were performed in triplicate. **P < 0.01. (E–H) U87MG and U251 cells were treated with LV‐Mock or LV‐PLAC2 and cell viability and cell cycle phase were evaluated with the CCK‐8 assay (E and G) and by FACS (F and H), respectively. PLAC2 overexpression in U87MG and U251 cells decreased cell proliferation and increased G1/S arrest relative to control cells. *P < 0.05, **P < 0.01.