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. 2017 Sep 18;22(1):497–510. doi: 10.1111/jcmm.13338

Figure 2.

Figure 2

PLAC2 overexpression inhibits cell proliferation and induces G1/S arrest via modulation of RPL36 expression. (A) RPL36 mRNA expression was up‐regulated in glioma as compared to normal brain tissues. *P < 0.05. (B) Fold change and correlation of PLAC2 and RPL36 mRNA levels in 55 glioma specimens by Spearman's correlation test. Relative fold change in expression was calculated with the 2−ΔΔCt method. *P < 0.05. (C, D) RPL36 mRNA (C) and protein (D) levels were markedly reduced by lentivirus‐mediated PLAC2 overexpression. Experiments were performed in triplicate (n = 3). *P < 0.05, **P < 0.01. (E–F) U87MG and U251 cells were treated with siRNA against RPL36, and cell viability and cell cycling were measured with the CCK‐8 assay (E) and by FACS (F), respectively. RPL36 knockdown inhibited proliferation and induced G1/S cell cycle arrest relative to control cells. Si‐1 and Si‐2 were the oligo used to knock down RPL36, Si‐RPL36 represents Si‐1; scrambled was the negative control. *P < 0.05, **P < 0.01. (G–H) Changes in the cell cycle after cotransfection of U87MG and U251 cells with indicated treatment. RPL36 overexpression in PLAC2‐overexpressing cells rescued G1/S arrest. *P < 0.05, **P < 0.01.