Figure 3.

DPDS 6 induces G₂/M cell cycle arrest in HTB‐54 cells. HTB‐54 cells were exposed to 2, 5, 10, 15, 20 and 30 μM DPDS 6 or vehicle for 24 hrs. After incubation, cells were processed for propidium iodide staining, and their cellular DNA content was determined by flow cytometry. Positive controls contained camptothecin at the same concentration as DPDS 6. (A) Flow cytometry analysis of a representative experiment. (B) The results expressed as percentages of total cell counts. (C) Time course analysis of cell cycle phase distribution after incubation of the cells with 10 μM DPDS 6. (D) Cell cycle phase distribution of A549 cell cultures after treatment with 30 μM DPDS 6 for 24 hrs. The results are the mean ± S.E.M. of three independent experiments performed in duplicate. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with control cells.