Figure 2.

Yes‐associated protein (YAP) knockout impairs neurite outgrowth. (A) Alignment of Sanger sequencing results of PCR amplicons encompassing the target site of YAP by CRISPR/Cas. SgRNAs targets are highlighted in red, and the PAM sequence is blue. Dashes, nucleotide deletions. (B) Western blotting analysis of YAP expression in WT and two KO cells. (C) Representative data of RA‐induced neurite outgrowth in WT cells or YAP KO cells. (D) Quantification of the percentage of neurite‐bearing cells in WT and KO cells. (E) Quantification of neurite length in WT and KO cells. Data show the Mean ± S.E.M. values of three replicates (*P < 0.05, **P < 0.001).