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. 2017 Aug 31;22(1):374–381. doi: 10.1111/jcmm.13324

Figure 5.

Figure 5

YAP stabilizes phosphorylated CREB upon RA treatment. (A) Endogenous CREB was immunoprecipitated from WT and YAP KO cells after treatment with MG132 to block degradation and probed for ubiquitination (Ub). (B) Western blotting of CREB in cells treated with RA and H89. (C) Western blotting of p‐CREB and CREB after transiently expressed YAP in knockout cells. (D) Quantification of the percentage of neurite‐bearing cells and average neurite length in KO cells after transfected with YAP or empty vector. Data show the Mean ± S.E.M. values of three replicates (*P < 0.05, **P < 0.001).