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. 2017 Aug 7;22(1):57–66. doi: 10.1111/jcmm.13292

Figure 1.

Figure 1

3′‐sialyllactose promotes and restores Col2a1 expression in articular chondrocytes. (A, B) Chondrocytes were treated with 3′‐sialyllactose in a dose‐dependent manner. Following 36 hrs of culture, Col2a1 was detected with RT‐PCR (A, upper panel), qRT‐PCR (A, lower panel), Western blot (B, upper panel) and densitometry (B, lower panel). (C) Articular cartilage explants were treated with 3′‐sialyllactose at the indicated dose. Following 72 hrs of explant culture, the accumulation of sulphated proteoglycans was assessed by Alcian blue staining. (D, E) Chondrocytes treated with IL‐1β (5 ng/ml) for 24 hrs were untreated or cotreated with 3′‐sialyllactose in a dose‐dependent manner. Col21 expression was determined by PCR (D, upper panel), qRT‐PCR (D, lower panel), Western blot (E, upper panel) and densitometry (E, lower panel). Gapdh and Erk were detected as loading controls. Values represent the means ± S.E.M. (*P < 0.05, **P < 0.0001). (F) Articular cartilage explants stimulated with IL‐1β were treated or not with 3′‐sialyllactose for 72 hrs. The accumulation of sulphated proteoglycans was determined by Alcian blue staining.