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. 2017 Aug 7;22(1):57–66. doi: 10.1111/jcmm.13292

Figure 6.

Figure 6

3′‐Sialyllactose regulates anabolic and catabolic factor expression via Erk and NF‐κB pathways. (A, B) Chondrocytes were pre‐treated with different concentrations of 3′‐sialyllactose for 24 hrs prior to treatment with IL‐1β (5 ng/ml) for 10 min. Erk phosphorylation was measured by Western blotting (A, upper panel) and densitometry (A, lower panel). The protein level of IκB was detected by Western blotting (B, upper panel) and densitometry (B, lower panel). Erk was detected as a loading control. (C) Immunohistochemical staining of Sox9, IκB and pErk in DMM‐induced OA mice with administration of PBS and 3′‐sialyllactose. (D) Schematic diagram of the signalling pathway leading to Sox9 activation and NF‐κB inhibition by 3′‐sialyllactose. Values represent the means ± S.E.M. *P < 0.05.