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. 2017 Aug 7;22(1):695–699. doi: 10.1111/jcmm.13315

Figure 2.

Figure 2

The effect of silencing carbonic anhydrase I (CA I) on the MDA‐MB‐231 breast carcinoma cell morphology and expression. (A) A light‐microscope image of MDA‐MB‐231 cells 48 hrs after siCA1 transfection compared to parental MDA‐MB‐231 cells transfected with Mock siRNA. Magnification 200x. (B) Gene expression analysis of silenced CA1 gene and selected genes responsible for basal lamina assembly (COL1A1—collagen type I alpha 1, COL4A4—collagen type IV alpha 4, LAMC2–laminin subunit gamma 2, CTHRC1—collagen triple helix repeat containing 1, WNT7B—Wingless‐Type MMTV Integration Site Family, Member 7B); reverse transcriptase quantitative PCR (RT‐qPCR). (C) Western blot analysis indicating reduced amount of CA I protein after CA1 mRNA silencing. Beta‐actin was used as a loading control.