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Transwell migration assay using AC‐1M88 choriocarcinoma–trophoblast cells with or without 50 ng/ml PROK1 (A) Representative microphotographs (40× magnification) were taken with a Leica DFC420 C digital camera on a Nikon Eclipse TS 100 inverted microscope and converted to greyscale image by Adobe Photoshop. (B) Total area of the migrated cells was analysed by ImageJ software. Values are presented as means and standard deviations. **P < 0.01.
