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. 2017 Dec 20;4(12):113. doi: 10.3390/children4120113

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Identification of minor population of erythroid leukemic cells (red dots), cluster of differentiation 45⁻ (CD45⁻), CD34^+/−, CD36⁺, CD71⁺ with abnormal expression of CD105, CD117, Histocompatibility Leukocyte Antigen-D Related (HLADR), and CD33. The correct characterization of these mild changes requires comparison with reference images obtained from normal counterpart (pink line); (B) Identification of blast cells in tissue biopsy. Standardized eight-color flow cytometry (FC) demonstrated a homogeneous population similar (not identical) to the previously identified population in a bone marrow sample. Comparison with reference images demonstrated alterations in the expression of CD36, CD71, CD33, and HLADR. The intense homogeneous positivity to glycophorin A demonstrates the pure erythroid nature of the process.