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Commonly used experimental approaches to study sublethal heat injury. (A) Differential plating technique in non-selective (■) and selective medium (▲): the shadowed area corresponds to the population with sublethal injuries. (B) Study of the growth dynamics of non-injured (■) and injured populations (▲) after heat treatment: the lag phase is longer in the latter. Nt: number of microorganisms at time t. N0: number of microorganisms at time 0. Abs: absorbance.
