Table 1.
Characterization of ALK fusion partners in NSCLC.
| Protein Name 1 | Reported ALK Fusion |
Uniprot ID | Molecular Function 2 | Subcellular Localization 3 | Protein Domains 4 | |
|---|---|---|---|---|---|---|
| Echinoderm microtubule-associated protein-like 4 (EML4) | E2a/b;A20 | [36] | Q9HC35 | Microtubule binding and assembly | Cytoplasm > cytoskeleton |
Coiled coil [aa17–51] HELP [aa225–297] WD40 repeats [aa229–347, 499–537, 583–620, 711–749, 824–863] |
| E6a/b;A20 | [32] | |||||
| E13;A20 | [20] | |||||
| E13b;A20 | [33] | |||||
| E14;A20 | [33,36] | |||||
| E15;A20 | [34] | |||||
| E18;A20 | [35] | |||||
| E20;A20 | [20] | |||||
| Kinesin-1 heavy chain (KIF5B) | K24;A20 K15;A20 |
[33] [43] |
P33176 | Microtubule-associated motor protein | Cytoplasm > cytoskeleton |
Kinesin [aa14–325] Coiled coil [aa330–364, 418–543, 594–684, 691–711, 716–757, 768–802, 825–912] |
| TRK-fused gene (TFG) | T3;A20 | [21] | Q92734 | Dynamic interaction of endoplasmic reticulum and microtubules | Endoplasmic reticulum (ER) |
PB1 [aa10–93] Coiled coil [aa102–122] |
| Dynactin subunit 1 (DCTN1) | D26;A20 | [37] | Q14203 | Dynein-mediated retrograde transport of vesicles and organelles along microtubules | Cytoplasm > cytoskeleton > microtubule |
CAP-Gly [aa29–94] Coiled coil [aa217–352, 360–383,388–540, 952–1043, 1185–1205] Dynactin [aa527–805] |
| Sequestosome-1 (SQSTM1) | S5;A20 | [37] | Q13501 | Autophagy receptor, endosome organization | Cytoplasm > late endosome, autolysosome/-phagosome |
PB1 [aa21–102] Zinc finger [aa122–165] UBA [aa379–440] |
| Nucleoprotein TPR | T15;A20 | [38] | P12270 | Scaffolding element in nuclear pore complex, nucleocytoplasmic transport | Nucleus > Nuclear pore complex |
Coiled coil [aa29–49, 61–172, 219–281, 293–366, 427–513, 543–570, 576–596, 665–752, 760–801, 827–868, 887–914, 934–982, 997–1071, 1096–1116, 1149–1169, 1221–1238, 1269–1303, 1311–1345, 1354–1416, 1469–1552, 1564–1598, 1603–1630] TPR/MLP1/MLP2 [aa1038–1165] |
| Cysteine-rich motor neuron 1 protein (CRIM1) | n/a | [26] | Q9NZV1 | Tissue development, interaction with transforming growth factor beta family proteins | Cell membrane |
Single pass type I transmembrane [aa1–34] IGFBP [aa37–90] VWC [aa336–390, 403–456, 608–662, 679–734, 753–808, 819–873] Antistatin [aa469–498, 505–532, 539–564, 567–592] |
| Striatin (STRN) | S3;A20 | [39] | O43815 | Calmodulin-binding protein involved in scaffolding and signaling | Cytoplasm, Cell membrane |
Striatin [aa48–177] Coiled coil [aa67–115] WD40 repeats [aa453–491, 506–544, 559–597, 696–734, 738–779] |
| Huntingtin-interacting protein 1 (HIP1) | H21;A20 | [40] | O00291 | Involved in clathrin-mediated endocytosis and trafficking | Cytoplasm, Endomembrane system, nucleus |
ANTH [aa39–308] Coiled coil [aa377–397, 409–488, 496–555, 584–611, 978–1007] Clathrin binding [aa482–580] I/LWEQ [aa862–1010] |
| Tyrosine-protein phosphatase non-receptor type 3 (PTPN3) | Chimeric fusion | [44] | P26045 | Tyrosine phosphatase, interaction with cytoskeleton | Cell membrane, cytoplasm > cytoskeleton |
FERM [aa33–97, 113–222, 226–316] PDZ [aa510–595] Y phosphatase [aa670–900] |
| Kinesin light chain 1 (KLC1) | K9;A20 | [41] | Q07866 | Microtubule-associated motor protein | Cytoplasm > cytoskeleton | n/a |
| Clathrin heavy chain 1 (CLTC) | C31;A20 | [22] | Q00610 | Intracellular trafficking and endocytosis | Cell membrane, cytoplasm > cytoskeleton |
Clathrin propeller repeat [aa19–56, 148–187, 198–234, 253–288, 296–330] Clathrin (H) link [aa331–354, 356–421] Clathrin [aa537–679, 686–827,835–971, 979–1123, 1128–1268, 1274–1419, 1424–1565] |
| F-box only protein 36 (FBXO36) | n/a | [42] | Q8NEA4 | Substrate recognition in E3 ubiquitin ligase complex | n/a |
F-box [aa92–139] Coiled coil [aa166–186] |
1,2,3 Information retrieved from UniProt database (http://www.uniprot.org), accessed on 9 April 2017 [85]; 4 Information retrieved from Pfam database (http://pfam.xfam.org), accessed on 9 April 2017 [52]. Protein domains are underlined and include coiled-coiled domain, hydrophobic EMAP-like protein (HELP) motif, WD40 repeats, kinesin domain, PB1 domain, cytoskeleton-associated protein (CAP)-Gly domain, dynactin motif, ZZ-type zinc finger domain, ubiquitin associated (UBA) domain, TPR/myosin-like protein (MLP) 1/2 motif, single pass type I transmembrane domain, insulin-like growth factor binding protein (IGFBP) motif, von Willebrand factor type C (VWC) domain, antistatin domain, striatin domain, ANTH domain, clathrin binding domain, I/LWEQ domain, FERM domain (F, 4.1 protein; E, ezrin, R, radixin; M, moesin), PDZ domain, Y phosphatase domain, clathrin propeller repeat, clathrin (H) link, clathrin domain, and F-box domain.