Figure 5. Podocalyxin regulates microvillus formation and cell spacing in mouse podocytes.
(A) Schematic of knockout mouse generation and analysis. (B) TEM images of podocytes from Podxl−/− prenatal mice (E18.5), or age-matched littermate controls. Images from two representative experiments are shown. Zoom is shown to the right for dashed boxed regions. m, microvilli; J, junctions. (C) Quantification of microvillus number and (D) cell separation in Podxl−/− podocytes and littermate controls, with associated p-values. Each point represents a single podocyte (n = 4 control and 5 Podxl−/− mice). Scale bars, 5 μm.