. Author manuscript; available in PMC: 2018 Jan 1.

Published in final edited form as: SLAS Discov. 2017 Aug 29;23(1):55–64. doi: 10.1177/2472555217728489

Table 1.

AmpG Absorbance 1536wpf Primary HTS protocol

Order	Step	Condition	Comments
1	Measure absorbance of PAO1 bacteria stock (overnight culture)	Optical Density	Dilute 1:10 in CAMHB and measure OD₆₀₀ on spectrophotometer
2	Dilute PAO1 to 10^6 CFU/mL		CAMHB + 200ug/mL Cefoxitin
3	Dispense CAMHB	2.5μL/well	Greiner black w/clear bottom 1536 well plate
4	Pin compounds/controls	100nL	Compounds pinned at 18.6uM final concentration in 2% DMSO
5	Dispense PAO1	2.5μl/well
6	Incubation	17 hours	37C in humidified chamber
7	Read Absorbance	Absorbance	600nm on Envision (Perkin Elmer)