Figure 1. Addition of dietary I3C reduces CDI-related mortality and morbidity.

(A) Diagram of CDI model testing efficacy of dietary I3C on disease progression. Treatments include addition of antibiotics (ABx) to drinking water, i.p injection of Clindamycin (CLI) and oral gavage with C. difficile spores. (B) Percent survival, (C) mean percent weight +/− SEM and (D) mean clinical score +/− SEM after inoculation with 10⁵ VPI 10463 C. difficile of male B6 mice fed standard chow or chow + I3C (1000 ppm) starting two weeks prior to ABx treatment. Data was pooled from two independent experiments. (E) Percent survival, (F) mean percent weight +/− SEM and (G) mean clinical score +/− SEM after inoculation with 10⁴ VPI 10463 C. difficile of male B6 mice fed semi-purified diet (diet) or semi-purified diet supplemented with I3C (1000 ppm) starting two weeks prior to ABx treatment. Data was pooled from three independent experiments. Daily mice at risk are indicated in panels B and F. Log-rank test was used for survival analysis, two-tailed Student’s t-test was used for comparing daily percent weight and Mann-Whitney U test was used for comparing daily clinical score. * P ≤ 0.05; ** P ≤ 0.01; *** P < 0.001.