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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Ann Surg. 2017 Jun;265(6):1183–1191. doi: 10.1097/SLA.0000000000001830

Figure 4. Mice fed I3C supplemented diet display increased neutrophil response to C. difficile without increased cecal inflammation.

Figure 4

(A) Cecal C. difficile CFU/g feces in diet and diet + I3C mice at day 2 and 4 (n=4 per group). Limit of detection indicated by dash-line. Determination of the presence or absence of C. difficile cfu for each day determined by Chi-square analysis. (B and C) Representative flow plots and % of cLP neutrophils per CD45+ cells on day 0 (n=4 per group) and day 3 (n=8 per group) after inoculation in diet and diet + I3C mice. (D and E) Representative histology at 100x and 400x in semi-purified diet and I3C mice at day 3 (arrows depict neutrophils) and corresponding histopathological scoring (n=10 per group). (F) Relative IL-22, IL-17, and IFN-γ expression at day 0 and 4 as measured by RT-PCR in semi-purified diet and diet + I3C mice normalized to β-actin (n=4–10 per group). (G) Measurement of total IL-22 and IL-17A protein content per cecum by ELISA.