Table 1.
Primer sequences used for real-time RT-PCR analyses
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| COL1α2 | TGGCAAGGCCGGTGAAGATA | TGACCAGGAAGCCCTCGGACT |
| RUNX2 | GCACCCAGCCCATAATAGA | TTGGAGCAAGGAGAACCC |
| ALP | CGCTTTGACCAACAGTGTGGA | CCTTGTAACCAGGCCCGTTG |
| IFT88 | TCAGGCTATTGAGTGGCT | TCTCGCAGAACTGGGTAT |
| AC1 | CCAACGTAATGACCTGTGAGGATG | AGGCGGCTGATGTACCTGTTG |
| AC2 | GCCAGCAGAATGGACAGCAC | GCCAAGCGTCTGCAAGATGA |
| AC3 | CCTGGGCCATGTTAGCCATC | TACAGCCACCGTCCAGCTCTATC |
| AC4 | AGCTGGTGCTAATGCTCAATGAA | CAATTGATGGCGTGGTCAGG |
| AC5 | TGCCATGGAGATGAAAGCAGA | TGGGATGCCAGGCTAGTGAAG |
| AC6 | AAGGACTCTAAGGCATTCCGACA | CCACCTCATCCTCAGGGTTCA |
| AC7 | CTGATGAGCAAGCTGGATGGAA | CTGATGAGCAAGCTGGATGGAA |
| AC8 | TGGAATCCATTCAGGCTCTGTG | CCAGTGTGGCTTTGGAAATGTG |
| AC9 | ACCTACCTTTACCCAAAGTGCACGGACAAT | CTCGGCGCTGCCTCACACACTCTTTGAGAC |
| AC10 | GTCACCTCCAGCATCGCTCA | AATGATAGCAGCACACCTCACCAG |
| GAPDH | TATCGGACGCCTGGTTAC | CTGTGCCGTTGA ACTTGC |