Table 1.

PCR primers used for determining which PMCA isoforms are present in mouse and human pancreatic acinar cells

F, forward; R, reverse.

Gene	GenBankTM accession number	Primer sequence	Expected size	Refs.
Mouse
PMCA1	NM_026482	F, 5′-TCT GGC TAC GGA ACC ACC CA-3′	390	4, 9
		R, 5′-AAA GGC TTC CCG CCA AAC TG-3′
PMCA2	NM_009723	F, 5′-CAC CCT GCT CTT CGT GGG TG-3′	355	4, 9
		R, 5′-AGG GAT GGT GGC GAT GAC CT-3′
PMCA3	NM_177236	F, 5′-ATG AAG AGC TGG CCG AAG GG-3′	345	4, 9
		R, 5′-GCG TGG TCA GGT AGA TGC CG-3′
PMCA4	NM_213616	F, 5′-CAA GCC TCT GAT CTC CCC CA-3′	364	4, 9
		R, 5′-CCA CTG CTC CAT GGT CAG GC-3′
PMCA4a	NM_001167949	F, 5′-GCA CTG GAT GTG GTG TCT CTT TAT-3′	417	11
		R, 5′-CAC TCA CTG CCC ACA GGA GGA-3′
PMCA4a/b	(a) NM_001167949/(b) NM_213616.3	F, 5′-GGA CGA GAT TGA CCT TGC CG-3′	(a) 469/(b) 278	11
		R, 5′-CAC CAT CCA ACA GGA GCA CAC T-3′
Human
PMCA1	NM_001001323.1	F, 5′-AGC ACT GTC TCA GAC CAA CG-3′	555	a
		R, 5′-AGG AGC ATG CAA AGG AGC AT-3′
PMCA2	NM_001001331.2	F, 5′-GAA ACC GTG CAG GAT TGG TG-3′	179	a
		R, 5′-GTG AGC TCC AAG AGG TGT CC-3′
PMCA3	NM_021949.3	F, 5′-GCT CTG GTG CCT GTT TGT TG-3′	157	a
		R, 5′-GAT CTC TTC CTC GCC TTC GG-3′
PMCA4a/b	(a) NM_001001396.2/(b) NM_001684.4	F, 5′-CCG CAA TAC CTA CCC GAT CC-3′	(a) 597/(b) 419	a
		R, 5′-GCA ATC CAC CGC ATT GTT GT-3′

^a Primers were designed using Primer-BLAST.