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. 2017 Oct 18;292(51):21071–21082. doi: 10.1074/jbc.M117.811448

Table 1.

Parameters for conversion of ThT fluorescence intensity to [Aβagg] (μm)

aa b
40 33 ± 8 25 ± 10
42 430 ± 35 44 ± 15

a Parameters a and b were determined by non-linear least-squares fitting of ThT fluorescence data at 72 h to ThT − ThTb = a[Aβ]0 + b[Aβ]02 (Equation 1), where [Aβ]0 is the total Aβ concentration in μm and [Aβagg] = [Aβ]0 at 72 h. ThTb was determined by averaging three sample wells containing ThT but no Aβ and was found to be 520 ± 40. Error estimates are 95% confidence intervals.