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. 2017 Oct 23;292(51):21092–21101. doi: 10.1074/jbc.M117.792598

Table 2.

Kinetic parameters of BlArap42B on plant glycosides and pNP-αl-Arap

Substrate Km kcat kcat/Km
mm s1 mm1 s1
Paeonolidea 0.074 ± 0.007 240 ± 10 3,200 ± 200
Ginsenoside Rb2a 0.051 ± 0.018 79 ± 8 1,500 ± 400
pNP-α-l-Arapb 3.4 ± 0.4 160 ± 10 46 ± 0.3

a Measured at 30 °C in 40 mm sodium citrate (pH 6.5).

b Measured at 37 °C in 40 mm sodium citrate (pH 6.5) and 0.005% Triton X-100.