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. 2017 Oct 26;292(51):21102–21116. doi: 10.1074/jbc.M116.774836

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — Mensacarcin treatment triggers cytochrome c release from mitochondria into the cytoplasm of melanoma cells. A, the cytosolic fractions of SK-Mel-5 cells non-treated or treated with mensacarcin (50 μm) and staurosporine (10 μm) for 6 h were analyzed for cytochrome c content by Western blot analysis. GAPDH levels served as loading control. B, densitometric measurement of Western blotting. Staurosporine control was set to 100% cytochrome c release. Results are presented as mean ± S.D. (error bars) of triplicates (n = 3). *, p < 0.1; ***, p < 0.001 versus DMSO control.