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. 2017 Oct 26;292(51):21102–21116. doi: 10.1074/jbc.M116.774836

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 9. — Mensacarcin impairs mitochondrial function in melanoma cells. A, OCR and ECAR were measured using the Seahorse analyzer XF24. After basal measurements, mensacarcin was injected at different concentrations (black arrows) (n = 3). B, OCR was measured after mensacarcin was injected (black arrow) in different concentrations, followed by consecutive injections of oligomycin (1 μm), FCCP (0.5 μm), and antimycin A (0.5 μm)/rotenone (0.5 μm) (n = 3). C, basal bioenergetics state of SK-Mel-28, SK-Mel-5, and HCT-116 cells (n = 25). Error bars, S.D. ***, p < 0.001 versus DMSO control.