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KIBRA knockdown podocytes are protected from protamine-induced injury. A, representative Western blot showing effective KIBRA knockdown in human podocytes via shRNA. B, after treatment with protamine sulfate, phalloidin staining reveals that KIBRA knockdown podocytes have preservation of actin stress fibers, whereas control podocytes have significant loss of actin fibers as compared with vehicle-treated cells. Scale bar, 100 μm.
