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TLC analysis of isolated 32P-lipids from deep-rough E. coli that express V. cholerae glycine modification components. Each lane represents lipid material from E. coli K12 W3110 ΔlpxMΔlpxTΔrfaDFCΔeptAΔeptB harboring pQlink plasmids that heterologously express V. cholerae proteins indicated alphabetically below the plate (N, E, F, G, or S) and as detailed in the box legend (bottom right).
