Figure 5.

MALDI-MS structural analysis of deep-rough E. coli that express V. cholerae glycine modification components. All spectra are from the same background strain of E. coli W3110 ΔlpxMΔlpxTΔrfaDFCΔeptAΔeptB. Lipids analyzed here correspond to strains from Fig. 4: W3110 ΔlpxMΔlpxTΔrfaDFCΔeptAΔeptB strains harboring pQlink plasmids expressing lpxN or lpxN, almEFG as indicated. MALDI-MS analyzed lipids were isolated from cultures grown without ³²P. Left y axis denotes relative intensity and the x axis is of the m/z range analyzed.