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Expression of single site AlmG alanine variants in V. cholerae ΔalmG strains. The determined MIC value is reported below each image. Strains tested include E7946 wild type (WT), a positive control, and E7946 ΔalmG strains harboring pQlink expression plasmids that contain no gene (negative control), wild-type copy (complemented), or single-site AlmG alanine variants as indicated above each image. Representative plates imaged from experiments performed in triplicate are shown.
