Figure 1.

Effect of quercetin on the expression levels of DSB repair related proteins.

Notes: (A) OV2008 cells were treated with quercetin at the indicated concentrations for 12 h. After incubation, cell extracts were analyzed by Western blotting. Actin was used as a loading control. (B) OV2008 cells were treated with vehicle, quercetin (100 μM), tunicamycin (5 μM), or quercetin (100 μM) in the presence of the ER stress inhibitor 4-PBA (10 μM) for the indicated times. After incubation, cell extracts were analyzed by Western blotting. Actin was used as a loading control. (C) OV2008 cells were treated with quercetin (100 μM) in the presence or absence of 4-PBA (10 μM), or the ER stress inducer tunicamycin (5 μM), for 12 h as indicated. After incubation, cell extracts were analyzed by immunofluorescence microscopy conducted using anti-Rad51 staining. Shown are mean foci per nucleus counted from three independent experiments. *p<0.05, when compared with control.

Abbreviations: 4-PBA, 4-phenyl butyrate; DAPI, dihydrochloride; DSB, double-strand break; ER, endoplasmic reticulum.