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. Author manuscript; available in PMC: 2017 Dec 26.
Published in final edited form as: Cell. 2016 Aug 11;166(5):1188–1197.e9. doi: 10.1016/j.cell.2016.07.033

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
anti-TERT rabbit monoclonal Abcam Ab32020
anti-Rabbit peroxidase-Affinipure Jackson Labs 711-035-152
anti-TRF2 mouse monoclonal Imgenex IMG-124A
anti-HA mouse monoclonal Abcam ab18181
anti-Coilin rabbit polyclonal Santa Cruz Biotech. sc-32860
anti-53BP1 rabbit polyclonal Novus Biologicals NB100-304
anti-Mouse Alexa 488 preabsorbed Abcam Ab150117
anti-Rabbit Alexa 405 Life Technologies A-31556
anti-Rabbit Alexa 647 Life Technologies A-21244
anti-TERT sheep polyclonal Cohen et al., 2007 N/A
Chemicals, Peptides, and Recombinant Proteins
JF646 HaloTag Ligand Grimm et al., 2015 N/A
JF646 SNAP-tag Ligand Grimm et al., 2015 N/A
Prolong Diamond Antifade Mountant Life Technologies P36970
Cell line Nucleofector Kit R Lonza VCA-1001
Puromycin 10 mg/ml Sigma P9620-10ML
Thymidine Sigma T1895
GlutaMAX™ Supplement ThermoFisher Scientific Cat # 35050061
SuperSignal® West Femto Chemiluminescent Substrate ThermoFisher Scientific Cat # 34095
Experimental Models: Cell Lines
HeLa EM2-11ht Weidenfeld et al., 2009 N/A
HeLa EM2-11ht, FLAG-HaloTag-TERT WT, HA-mEOS3.2-TRF2 This Paper N/A
HeLa EM2-11ht, FLAG-HaloTag-TERT K78E, HA-mEOS3.2-TRF2 This Paper N/A
HeLa EM2-11ht, FLAG-SNAP-TERT Xi et al., 2015 N/A
Recombinant DNA
pHTN HaloTag® CMV-neo Promega G7721
pFastBac1 Life Technologies 10360014
FLAG-LoxP-PURO-LoxP-SNAP-TERT HR Donor Xi et al., 2015 Addgene: 71390
FLAG-LoxP-PURO-LoxP-HaloTag-TERT WT HR Donor This Paper N/A to be deposited
FLAG-LoxP-PURO-LoxP-HaloTag-TERT K78E HR Donor This Paper N/A to be deposited
LAP-BFP-Coilin Cheeseman et al., 2005 N/A to be deposited
pX330 Cong et al., 2013 Addgene: 42230
mEos3.2-N1 Zhang et al., 2012 Addgene: 54525
HA-mEOS3.2-TRF2 HR Donor This Paper N/A to be deposited
BFP-Nucleolin Takagi et al., 2005 Addgene: 28176
Sequence-Based Reagents
sgRNA SNAP1: CATTTCGCAATCTTTGTCCA This Paper N/A
sgRNA SNAP2: GGTATAGGCGCGCCAGGATC This Paper N/A
TRF2 PCR oligo 1 for Gibson Assembly into pFastBac1: CATAAAATGAATGCAATTGTTGTTGTTGGCCACCTGCACTTCTGAGTTGT This Paper N/A
TRF2 PCR oligo 2 for Gibson Assembly into pFastBac1: CTGGAACATCGTATGGGTACATCGTGTCCGATCGCCGCGCG This Paper N/A
TRF2 PCR oligo 3 for Gibson Assembly into pFastBac1: CAATGCCAGACGAGGATCCATGGCGGGAGGAGGCGGGAGTAG This Paper N/A
TRF2 PCR oligo 4 for Gibson Assembly into pFastBac1: CATTATAAGCTGCAATAAACAAGTTCCCATCCTCCTCAGATACGAGTGGCAAG This Paper N/A
mEOS3.2 PCR oligo 1 for Gibson Assembly into pFastBac1: ATGTACCCATACGATGTTCCAGATTACGCTATGAGTGCGATTAAGCCAGACATG This Paper N/A
mEOS3.2 PCR oligo 2 for Gibson Assembly into pFastBac1: GGATCCTCGTCTGGCATTGTCAGGCAATC This Paper N/A
HaloTag PCR Oligo 1: GCGCGCGGTACCATGGCAGAAATCGGTACTGGCTTTCC This Paper N/A
HaloTag PCR Oligo 2: GCGCGCGGTACCGGAAGCGATCGCGTTATCGC This Paper N/A
Coilin PCR Oligo 1: GCGCGCCTCGAGATGGCAGCTTCCGAGACGGTTAG This Paper N/A
Coilin PCR Oligo 2: GCGCGCGGTACCTCAGGCAGGTTCTGTACTTGATGTGTTAC This Paper N/A
Coilin Image Clone GE Dharmacon CloneID: 4275993
Software and Algorithms
MatLab2011b Mathworks Inc., USA http://mathworks.com
SLIMfast MatLab script Liu et al., 2014; Serge et al., 2008 Provided by Zhe Liu liuz11@janelia.hhmi.org
evalSPT MatLab script Liu et al., 2014; Normanno et al., 2015 Provided by Zhe Liu liuz11@janelia.hhmi.org
DiffusionSingle MatLab script Liu et al., 2014 Provided by Zhe Liu liuz11@janelia.hhmi.org
MSDanalyzer MatLab script Tarantino et al., 2014 https://www.mathworks.com/matlabcentral/fileexchange/40692-mean-square-displacement-analysis-of-particles-trajectories
Other
TetraSpeck™ microspheres ThermoFisher Scientific T-7279
NuPAGE NOVEX 4-12% Bis-Tris Protein Gels ThermoFisher Scientific Cat # NP0321BOX
Protein G Agarose Roche Cat # 11719416001
ANTI-FLAG® M2 Affinity Sigma A2220