Figure 7.

Comprehensive immunohistologic analysis of carotid and aortic plaques. (A) Representative images of immunohistochemical staining and fluorescence microscopy of carotid plaques. (B) Lipid contents were assessed by Oil Red O (ORO) staining. (C) Macrophage contents were analyzed by Mac3 immunostaining. Data are presented as mean ± SD. n = 6 mice per group; *P < 0.05, ** P < 0.01, by ANOVA followed by Mann-Whitney test. (D) Representative images of the aortic root lesions subjected to hematoxylin and eosin (H&E) staining, ORO staining, immunostaining for pan-macrophage marker Mac3 and classically activated macrophage marker CD86 in each group. Quantification results of H&E (E), ORO (F), Mac3 (G), and CD86 (H) stained area. Data are presented as mean ± SD. n = 6 mice per group; * P < 0.05, ** P < 0.01, by ANOVA followed by Mann-Whitney test.