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. 2018 Jan 1;8(1):109–123. doi: 10.7150/thno.20356

Figure 6.

Figure 6

PrLZ/LKB1-mediated autophagy is involved in docetaxel-induced apoptosis in PCa cells. (A) Small interference RNA against LKB1 (si-LKB1) was transfected into C4-2 cells for 48 h. The expressions of autophagy- and apoptosis-related molecules were assayed by western blotting in C4-2 cells treated with docetaxel (DTX) (20 μM, 24 h). Under similar conditions, the GFP-RFP-LC3 puncta (B), cell viability (C) and percentage of apoptotic cells (D) were quantified in DTX-treated LKB1 knockdown (si-LKB1) and control C4-2 cells. The results represent the mean ± S.D. of 3 independent experiments. *, P<0.05, **, P<0.01, ***, P<0.001 and ****, P<0.0001. (E) The expression levels of autophagy- and apoptosis-related molecules were determined by western blotting in PrLZ knockdown (sh-PrLZ) cells transfected with LKB1 siRNA (si-LKB1). (F) Small interference RNA against ATG5 (si-ATG5) was transfected into C4-2 cells for 48 h. The expression levels of apoptosis markers and the LC3-Ⅱ/ LC3-Ⅰ ratio were determined by western blotting in C4-2 cells treated with docetaxel (DTX) (20 μM, 24 h). Under similar treatment conditions, the GFP-RFP-LC3 puncta (G), cell viability (H) and percentage of apoptotic cells (I) were quantified in docetaxel-treated C4-2 cells. The results represent the mean ± S.D. of 3 independent experiments. **, P<0.01, ***, P<0.001 and ****, P<0.0001.