Figure 2.

Fluorescence image of (A) early stage PSC-CM (day ~30) and (B) late stage PSC-CM (day ~100). Stained for α-actinin and phalloidin, indicating an increase in sarcomeric organization and myofibril density with increased time in culture. Scale bar 25 μm ⁸¹. Reproduced with permission from John Wiley and Sons. (C) Median, maximum, and minimum sarcomere distance of human fetal cardiomyocytes (hFetal-CM), and hiPSC-CM and hESC-CM in standard BPEL media, cardiomyocyte media (CA), and maturation media (MM). ns = non-significant; *P < 0.05; **P < 0.01; ***P < 0.001 ⁴⁷. Reproduced with permission from Elsevier. (D) Late stage PSC-CM (day ~100) were fixed, stained, and imaged using fluorescence microscopy then compared to early stage PSC-CM (day ~30) for (E) %multinucleation, (F) sarcomere length, (G) cell perimeter, (H) cell area, and (I) circularity ⁸¹. Reproduced with permission from John Wiley and Sons.