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. 2018 Jan 1;8(1):124–140. doi: 10.7150/thno.19441

Figure 5.

Figure 5

(A) Genetic profile of early stage (~30 days) hESC-CM and hiPSC-CM, late stage (~100 days) hESC-CM and hiPSC-CM, and the adult human heart. Late stage cardiomyocytes show upregulation of MYH7 (β-myosin heavy chain), MYH6 (α-myosin heavy chain), GJA1 (connexin-43), HCN4 (hyperpolarization-activated K+ channel), and SERCA (sarcoendoplasmic reticulum ATPase). *P < 0.05 versus corresponding early-stage PSC-CMs. ∔P <0.05 versus adult human heart 81. Reproduced with permission from John Wiley and Sons. (B-G) Engineered human tissue (EHT) developed from neonatal rat cardiomyocytes compared to fetal, neonatal, and adult cardiomyocytes. Expression of (B) α-cardiac actin, (C) α-skeletal actin, (D) α-sarcomeric protein, (E) α-myosin heavy chain (MHC), (F) β-MHC, (G) α-/β-MHC. Note in the murine system α-MHC is predominantly present in the adult heart while β-MHC has a greater presence in the fetal heart. In the human system both α-MHC and β-MHC have increased expression in the adult heart. *P < 0.05 versus EHT day 0 (B, C, F, and G) and EHT day 3 (D) or between indicated columns (C, E, and G); ANOVA followed by Bonferroni multiple comparison test 106. Reproduced with permission from Wolters Kluwer Health, Inc.