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. 2018 Jan 1;8(1):256–276. doi: 10.7150/thno.22764

Figure 3.

Figure 3

Antiretroviral and MRI relaxivity measurements. Antiretroviral activity was determined in MDM treated for 8 h with free DTG, EuCF-PCL, EuCF-DTG or FA-EuCF-DTG nanoparticles (6.25, 12.5 and 25 µM DTG) and then infected with HIV-1ADA at a multiplicity of infection (MOI) of 0.1 at day 1 after drug loading. At 10 days after infection, progeny HIV virion production was determined by RT activity in the cell culture fluids. (A) HIV replication was determined 10 days after infection by HIV RT activity of day 1. Statistical differences were determined using one-way ANOVA among groups; we used Tukey's test to correct for multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001. (B) HIV p24 staining (scale bar = 200 μm). (c-d) MRI signal enhancement effects of EuCF-DTG and FA-EuCF-DTG nanoparticles were determined by calculating nanoparticle relaxivity r2 (mM-1s-1) in both PBS (extracellular) and MDM (intracellular) using a 7T MRI scanner. (C) Nanoparticle relaxation rates (R2) in both PBS and MDM increased linearly with increasing iron concentrations. (D) T2-weighted images of EuCF-DTG nanoparticles in PBS demonstrate signal reduction with increasing concentrations of iron.