Table 1.
Calculated fitting parameters of the fluorescence polarization data of DOX and 7-de-aDOX binding (Figure 4). Ki refers to the inhibition of DOX or 7-de-aDOX by either AA or EBS as indicated. Error represents the SEM of 3 experiments.
| Summary of Binding Parameters
|
||||
|---|---|---|---|---|
| r0 | r1 | KD (μM) | Ki (μM) | |
| DOX | ||||
| DOX | 0.083 ± 0.007 | 0.219 ± 0.010 | 5.32 ± 0.89 | — |
| DOX + AA | 0.094 ± 0.005 | — | — | 176 ± 64 |
| DOX + EBS | 0.101 ± 0.003 | — | — | 13.8 ± 1.6 |
| 7-de-aDOX | ||||
| 7-de-aDOX | 0.055 ± 0.006 | 0.351 ± 0.022 | 13.1 ± 1.1 | — |
| 7-de-aDOX + AA | 0.084 ± 0.002 | — | — | 210 ± 26 |
| 7-de-aDOX + EBS | 0.159 ± 0.006 | 0.383 ± 0.021 (app) | 13.4 ± 1.4 (app) | ∞ |