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. 2018 Jan 1;8(2):423–436. doi: 10.7150/thno.22377

Figure 3.

Figure 3

DNA methylation leads to downregulation of miR-193a-3p during the tumor development and progression of ovarian cancer cells. (A) qPCR analysis revealing a stepwise decrease in miR-193a-3p expression from Stages 1 to 4 and Grades 1 to 3 in ovarian cancers. (B) Representative photographs showing an inverse correlation between miR-193a-3p and GRB7 expression by ISH and IHC analyses for serous subtype ovarian cancer samples (early stage = Stage 1 and Grade 2, and advanced stage = Stage 2 and Grade 3) on a commercial ovarian cancer tissue array (OVC1021, Pantomics). Scale bar, 100 μm. (C) The expression of miR-193a-3p could be restored in ovarian cancer cells upon treatment with 5-Aza-dc (5 μM) for 4 days. (D) Verification of miR-193a-3p promoter methylation by MS-PCR in ovarian cancer cells upon 5-Aza-dC treatment. Treatment with 5-Aza-dC (5 μM) for 3-4 days. (E) Pyrosequencing analysis showing a progressive increase in DNA methylation with the tumor stage (Upper) and grade (Lower) of ovarian cancers.