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. 2018 Jan 1;8(2):575–592. doi: 10.7150/thno.21648

Figure 6.

Figure 6

circHECTD1-mediated HECTD1 expression regulates RAW264.7 cell activation in response to SiO2 exposure. (A) As shown in the qRT-PCR analysis, circHECTD1 lentivirus transfection did not affect the expression of HECTD1 mRNA in RAW264.7 cells (n=5); *P<0.05 vs. 0 h. (B) Representative western blot showing the effects of circHECTD1 lentivirus transfection on SiO2-induced HECTD1 expression in RAW264.7 cells. (C) Densitometric analyses of five separate experiments suggested that circHECTD1 lentivirus transfection attenuated the SiO2-induced increase in HECTD1 expression in RAW264.7 cells (n=5); *P<0.05 vs. the control group; #P<0.05 vs. the SiO2 group. (D) Representative western blot showing that transfection with ACT or NIC upregulated and downregulated HECTD1 expression, respectively, in RAW264.7 cells. (E) Densitometric analyses of five separate experiments suggested that transfection with HECTD1 ACT and NIC upregulated and downregulated HECTD1 expression, respectively, in RAW264.7 cells (n=5); *P<0.05 vs. the control group. (F) Representative western blot showing the effects of ACT and NIC transfection on NOS2, ARG1 and SOC3 expression in RAW264.7 cells. (G) Densitometric analyses of five separate experiments suggested that HECTD1 ACT and NIC transfection affected NOS2, ARG1 and SOC3 expression in RAW264.7 cells (n=5); *P<0.05 vs. the corresponding control group. (H) Representative western blot showing the effects of HECTD1 ACT and NIC transfection on SiO2-induced NOS2 expression in RAW264.7 cells. (I) Densitometric analyses of five separate experiments suggested that SiO2-induced NOS2 expression was enhanced by HECTD1 ACT transfection but attenuated by HECTD1 NIC transfection. *P<0.05 vs. the corresponding control group. (J) Representative western blot showing the effects of HECTD1 ACT and NIC transfection on SiO2-induced ARG1 and SOCS3 expression in RAW264.7 cells. (K) Densitometric analyses of five separate experiments suggested that SiO2-induced ARG1 and SOCS3 expression was enhanced by HECTD1 ACT transfection but attenuated by HECTD1 NIC transfection. *P<0.05 vs. the corresponding control group. (L) MTT assay showing the effect of HECTD1 ACT and HECTD1 NIC transfection on the viability of RAW264.7 cells (n=5); *P<0.05 vs. the corresponding control group.