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Steady-state kinetic analysis of extension past T and Tg (7 and 8).a
| X | dNTP | kcat (× 10−2 s−1) | Km (μM) | kcat/Km (× 10−2 s−1/μM) | Finsb |
|---|---|---|---|---|---|
| T (7) | T | 35.2 ± 7.5 | 1.0 ± 0.4 | 35.2 | ---- |
| Tg (8) | T | 1.1 ± 0.1 | 488 ± 24 | 0.002 | 1 |
| Tg (8) | G | 2.4 ± 0.03 | 177 ± 12 | 0.01 | 5.8 |
Data are the average ± std. dev. of two independent experiments performed in triplicate. bFins = (kcat/Km)dNTP/(kcat/Km)dATP
