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Fidelity of Tg bypass by Pol θ
| Template nucleotide | #Colonies sequenced | dA opposite X | Full-length extension | One-nucleotide deletion | Two-nucleotide deletion |
|---|---|---|---|---|---|
| T (9) | 15 | 15 (100%) | 15 (100%) | 0 | 0 |
| Tg (10) | 36 | 35 (97%)a | 8 (22%) | 2 (6%) | 26 (72%) |
Misinsertion of T accounted for one full-length bypass event
